Device

Part:BBa_K199131

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-03-29)


Lux positive feedback loop with 2 sites

LuxR is constantly produced under the control of the pLac promoter. LuxI is produced and aids in the conversion of 30C6. This activates the pLux promoter, creating a positive feedback loop. Large amounts of RFP are produced- cells should be bright red when pelleted even when the cultures are not induced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 483
    Illegal AgeI site found at 1426
    Illegal AgeI site found at 1538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 135


[edit]
Categories
Parameters
n/aLux positive feedback loop with 2 sites